Applications/ABySS

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Application Details

  • Description: ABySS is a de novo sequence assembler.
  • Version: 1.5.2 (gcc-4.9.3)
  • Modules: abyss/1.5.2/gcc-4.9.3
  • Licence: Free, open-source

Introduction

ABySS is a de novo sequence assembler intended for short paired-end reads and genomes of all sizes.

Usage Examples

Assemble a small synthetic data set


[username@login] module add abyss/1.5.2/gcc-4.9.3
[username@login] wget http://www.bcgsc.ca/platform/bioinfo/software/abyss/releases/1.3.4/test-data.tar.gz
[username@login] tar xzvf test-data.tar.gz
[username@login} abyss-pe k=25 name=test \
    in='test-data/reads1.fastq test-data/reads2.fastq'

Calculate assembly contiguity statistics


[username@login] module add abyss/1.5.2/gcc-4.9.3
[username@login] abyss-fac test-unitigs.fa

Parallel processing

The np option of abyss-pe specifies the number of processes to use for the parallel MPI job. This will allow you to use multiple cores on a single machine without any MPI configuration. To use multiple machines for assembly, you must create a host file for mpirun, which is described on the mpirun man page.

Do not run mpirun -np 8 abyss-pe. To run ABySS with 8 threads, use abyss-pe np=8. The abyss-pe driver script will start the MPI process, like so: mpirun -np 8 ABYSS-P.

The paired-end assembly stage is multithreaded but must run on a single machine. The number of threads to use may be specified with the parameter j. The default value for j is the value of np.

Note: this example is done on a high memory node, usually access would be achieved with the scheduler


[username@login01 ~]$ interactive -phighmem
salloc: Granted job allocation 3619680
Job ID 3619680 connecting to c231, please wait...
c231.vc-main

[username@c230 ~]$ module add abyss/1.5.2/gcc-4.9.3
[username@c230 ~]$ mpirun abyss-pe np=40

Through SLURM this would become the script:

#!/bin/bash

#SBATCH -J abyss
#SBATCH -p highmem
#SBATCH -N 2
#SBATCH --ntasks-per-node=40
#SBATCH -o %N.%j.%a.out
#SBATCH -e %N.%j.%a.err
#SBATCH -p highmem
#SBATCH --exclusive
#SBATCH -t 00:30:00

module purge
module add abyss/1.5.2/gcc-4.9.3

#Run your ABySS commands

abyss-pe name=test k=48 n=8 in='test-1.fa test-3.fa'

The Quantum Package has been designed pretty much as an interactive environment for quantum-chemistry calculations, in order to facilitate the user experience.

  • qp
  • qp_e_conv_fci
  • qp_name
  • qp_reset
  • qp_stop
  • qpsh
  • qp_convert_output_to_ezfio qp_export_as_tgz
  • qp_plugins
  • qp_set_frozen_core
  • qp_test
  • qp_create_ninja
  • qp_mpirun
  • qp_prepend_export
  • qp_srun
  • qp_update

Further Information






Modules | Main Page | Further Topics