Difference between revisions of "Applications/Ncbi-blast"

Revision as of 13:24, 5 April 2017

Application Details

Description: NCBI-Blast finds regions of similarity between biological sequences. The program compares nucleotide or protein sequences to sequence databases and calculates the statistical significance.
Version: 2.4.0
Modules: ncbi-blast/2.4.0
Licence: Open-source (BLAST is a registered trademark of the National Library of Medicine)

Usage Examples

Command Set

ncbi-blast provides the following commands:

blast_formatter
blastdb_aliastool
blastdbcheck
blastdbcmd
blastn
blastp
blastx
convert2blastmask
deltablast
dustmasker
legacy_blast.pl
makeblastdb
makembindex
makeprofiledb
psiblast
rpsblast
rpstblastn
segmasker
tblastn
tblastx
update_blastdb.pl
windowmasker

SLURM Script


#!/bin/bash

fasta=/home/user1/maker.all.proteins.fasta
seqs_per_file=300
files_per_dir=100
BLAST_DB=/home/user1/database/nr_metazoa
prefix=Tcancriformis_maker2
threads_per_job=28
e=1e-5
cul=10
n_seqs=50
fmt=5 #5=xml
basedir=$(pwd)
partition_script=/home/user1/ectools/partition.py

###########

echo -e "\nsplitting up files\n"
#cat $fasta | sed 's/ .*//g' > $genome.fasta
python $partition_script $seqs_per_file $files_per_dir $fasta

count=$(ls -1 | grep -E "^[0-9]{4}" |wc -l)
for i in $(seq $count -1 1)
do
        current=$(printf "%04d" $i)
        echo -e "processing directory $current\n"
        cd $current

        for p in $(ls -1 | grep -E "p[0-9]{4}$" | sort -nr)
        do

                echo -e "#!/bin/bash
#SBATCH -J b-$current-$p-$prefix
#SBATCH -N 1
#SBATCH --ntasks-per-node $threads_per_job
#SBATCH -o job-%j.out
#SBATCH -e job-%j.out
#SBATCH -p compute

#LOAD MODULE
module load ncbi-blast/2.4.0
#
date
cd $basedir/$current

echo -e \"\\\nNumber of scaffolds to process:\\\t\$(cat $basedir/$current/${p} | grep \">\" | wc -l)\"
echo -e \"\\\nTotal length of scaffolds:\\\t\$(cat $basedir/$current/${p} | grep \">\" -v | perl -ne 'chomp; print \"\$_\"' | wc -m)\\\n\"

blastp -query ${p} -db $BLAST_DB -outfmt $fmt -max_target_seqs $n_seqs -culling_limit $cul -num_threads \$SLURM_NTASKS_PER_NODE -evalue $e -out ${p}-vs-nt-n$n_seqs.cul$cul.$e.blastp.out.xml

echo -e \"\\\nDONE\\\n\"
date" > run_blastn_${p}.slurm.sh
                sbatch run_blastn_${p}.slurm.sh
        done
        cd ..
done

Further Information

https://blast.ncbi.nlm.nih.gov/Blast.cgi

@@ Line 10: / Line 10: @@
 ===Command Set===
-{| class="wikitable"
+ncbi-blast provides the following commands:
-| style="width:50%" | <Strong></Strong>
-| style="width:50%" | <Strong></Strong>
-|-
-| [[Applications|Applications]]
-| How to use the application modules that are available on Viper.
-|-
-| [[General|General]]
-| General information about using Viper.
-|-
-| [[Training|Training]]
-| Training information about how to extend your knowledge of using Viper.
-|-
-| [[Programming|Programming]]
-| Programming support on writing and compiling programs in a range of different languages available.
-|-
-|}
-blastdb_aliastool
-blastdbcmd
-blastn
-blastx
-deltablast
-legacy_blast.pl
-makembindex
-psiblast
-rpstblastn
-tblastn
-update_blastdb.pl
-blastdbcheck
-blast_formatter
-blastp
-convert2blastmask
-dustmasker
-makeblastdb
-makeprofiledb
-rpsblast
-segmasker
-tblastx
-windowmasker
+* blast_formatter
+* blastdb_aliastool
+* blastdbcheck
+* blastdbcmd
+* blastn
+* blastp
+* blastx
+* convert2blastmask
+* deltablast
+* dustmasker
+* legacy_blast.pl
+* makeblastdb
+* makembindex
+* makeprofiledb
+* psiblast
+* rpsblast
+* rpstblastn
+* segmasker
+* tblastn
+* tblastx
+* update_blastdb.pl
+* windowmasker
+===SLURM Script===
 <pre style="background-color: #f5f5dc; color: black; font-family: monospace, sans-serif;">
-[username@login] module load abyss/1.5.2/gcc-4.9.3
+#!/bin/bash
-[username@login] wget http://www.bcgsc.ca/platform/bioinfo/software/abyss/releases/1.3.4/test-data.tar.gz
-[username@login] tar xzvf test-data.tar.gz
-[username@login} abyss-pe k=25 name=test \
-    in='test-data/reads1.fastq test-data/reads2.fastq'
-</pre>
+fasta=/home/user1/maker.all.proteins.fasta
+seqs_per_file=300
+files_per_dir=100
+BLAST_DB=/home/user1/database/nr_metazoa
+prefix=Tcancriformis_maker2
+threads_per_job=28
+e=1e-5
+cul=10
+n_seqs=50
+fmt=5 #5=xml
+basedir=$(pwd)
+partition_script=/home/user1/ectools/partition.py
-===Calculate assembly contiguity statistics===
+###########
-<pre style="background-color: #f5f5dc; color: black; font-family: monospace, sans-serif;">
+echo -e "\nsplitting up files\n"
+#cat $fasta | sed 's/ .*//g' > $genome.fasta
-[username@login] module load abyss/1.5.2/gcc-4.9.3
+python $partition_script $seqs_per_file $files_per_dir $fasta
-[username@login] abyss-fac test-unitigs.fa
-</pre>
-===Parallel processing===
+count=$(ls -1 | grep -E "^[0-9]{4}" |wc -l)
+for i in $(seq $count -1 1)
+do
+        current=$(printf "%04d" $i)
+        echo -e "processing directory $current\n"
+        cd $current
-The np option of abyss-pe specifies the number of processes to use for the parallel MPI job. Without any MPI configuration, this will allow you to use multiple cores on a single machine. To use multiple machines for assembly, you must create a hostfile for mpirun, which is described in the mpirun man page.
+        for p in $(ls -1 | grep -E "p[0-9]{4}$" | sort -nr)
+        do
-Do not run '''mpirun -np 8 abyss-pe'''. To run ABySS with 8 threads, use '''abyss-pe np=8'''. The abyss-pe driver script will start the MPI process, like so: '''mpirun -np 8 ABYSS-P.'''
+                echo -e "#!/bin/bash
+#SBATCH -J b-$current-$p-$prefix
+#SBATCH -N 1
+#SBATCH --ntasks-per-node $threads_per_job
+#SBATCH -o job-%j.out
+#SBATCH -e job-%j.out
+#SBATCH -p compute
-The paired-end assembly stage is multithreaded, but must run on a single machine. The number of threads to use may be specified with the parameter j. The default value for j is the value of np.
+#LOAD MODULE
+module load ncbi-blast/2.4.0
+#
+date
+cd $basedir/$current
-'''Note''': this example is done on a high memory node, usually access would be achieved with the scheduler
+echo -e \"\\\nNumber of scaffolds to process:\\\t\$(cat $basedir/$current/${p} | grep \">\" | wc -l)\"
+echo -e \"\\\nTotal length of scaffolds:\\\t\$(cat $basedir/$current/${p} | grep \">\" -v | perl -ne 'chomp; print \"\$_\"' | wc -m)\\\n\"
-<pre style="background-color: black; color: white; border: 2px solid black; font-family: monospace, sans-serif;">
+blastp -query ${p} -db $BLAST_DB -outfmt $fmt -max_target_seqs $n_seqs -culling_limit $cul -num_threads \$SLURM_NTASKS_PER_NODE -evalue $e -out ${p}-vs-nt-n$n_seqs.cul$cul.$e.blastp.out.xml
-[username@c230 ~]$ module add abyss/1.5.2/gcc-4.9.3
+echo -e \"\\\nDONE\\\n\"
-[username@c230 ~]$ mpirun abyss-pe np=40
+date" > run_blastn_${p}.slurm.sh
+                sbatch run_blastn_${p}.slurm.sh
+        done
+        cd ..
+done
 </pre>
-Through '''SLURM''' this would become the script:
-<pre style="background-color: black; color: white; border: 2px solid black; font-family: monospace, sans-serif;">
-#!/bin/bash
-#SBATCH -J abyss
-#SBATCH -p highmem
-#SBATCH -N 2
-#SBATCH --ntasks-per-node=40
-#SBATCH -o %N.%j.%a.out
-#SBATCH -e %N.%j.%a.err
-#SBATCH --exclusive
-#SBATCH -t 00:30:00
-module purge
-module load abyss/1.5.2/gcc-4.9.3
-#Run your ABySS commands
-abyss-pe name=test k=48 n=8 in='test-1.fa test-3.fa'
-</pre>
 ==Further Information==
-* [http://computing.bio.cam.ac.uk/local/doc/abyss.html http://computing.bio.cam.ac.uk/local/doc/abyss.html]
+* [https://blast.ncbi.nlm.nih.gov/Blast.cgi https://blast.ncbi.nlm.nih.gov/Blast.cgi]
 {|

HPC

Difference between revisions of "Applications/Ncbi-blast"

Revision as of 13:24, 5 April 2017

Contents

Application Details

Usage Examples

Command Set

SLURM Script

Further Information

Navigation

Support Areas

Research

Tools