Difference between revisions of "Applications/Samtools"
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* [[applications/Cufflinks|Cufflinks]] | * [[applications/Cufflinks|Cufflinks]] | ||
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Latest revision as of 10:58, 16 November 2022
Application Details
- Description: The OBITools package is a set of programs specifically designed for analysing NGS data in a DNA meta-barcoding context, taking into account taxonomic information
- Version: 1.3.1.
- Module: module samtools/gcc/1.3.1.
- Licence: BSD License, MIT License
Usage Areas
Samtools is a suite of programs for interacting with high-throughput sequencing data. It consists of three separate repositories:
- Samtools - Reading/writing/editing/indexing/viewing SAM/BAM/CRAM format
- BCFtools - Reading/writing BCF2/VCF/gVCF files and calling/filtering/summarising SNP and short indel sequence variants
- HTSlib - A C library for reading/writing high-throughput sequencing data
With linkages to the following modules too:
Usage Examples
Some usage examples of Samtools are listed below:
Index the reference FASTA.
[username@login01 ~]$ samtools faidx ex1.fa
Convert the (headerless) SAM file to BAM. Note if we had used "samtools view -h" above to create the ex1.sam.gz then we could omit the "-t ex1.fa.fai" option here.
[username@login01 ~]$ samtools view -S -b -t ex1.fa.fai -o ex1.bam ex1.sam.gz
Build an index for the BAM file:
[username@login01 ~]$ samtools index ex1.bam
View a portion of the BAM file:
[username@login01 ~]$ samtools view ex1.bam seq2:450-550
Visually inspect the alignments at the same location:
[username@login01 ~]$ samtools tview -p seq2:450 ex1.bam ex1.fa
View the data in pileup format:
[username@login01 ~]$ samtools mpileup -f ex1.fa ex1.bam
Generate an uncompressed VCF file of variants:
[username@login01 ~]$ samtools mpileup -vu -f ex1.fa ex1.bam > ex1.vcf
Generate a compressed VCF file of variants:
[username@login01 ~]$ samtools mpileup -g -f ex1.fa ex1.bam > ex1.bcf
Module
[username@login01 ~]$ module samtools/gcc/1.3.1