Difference between revisions of "Applications/Bridger"

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==Usage Examples==
 
==Usage Examples==
  
Bridger is an efficient de novo trascriptome assembler for RNA-Seq data. It can assemble all transcripts from short reads (single or paired) without using a reference.  The software expects as input RNA-Seq reads in fasta or fastq format, and ouput all assembled candidate transcripts in fasta format. Briefly, it works in two step: first,  Bridger partitions the sequence data into many individual splicing graphs, each capturing the full transcriptional complexity at a given gene or no more than a few genes.  
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Celera Assembler is a de novo whole-genome shotgun ('''WGS''') DNA sequence assembler. It reconstructs long sequences of genomic DNA from fragmentary data produced by whole-genome shotgun sequencing.
  
Then,  Bridger uses a rigorous mathematical model called minimum path cover to search minimal set of paths(transcripts) that can be supported by our data and could explain all observed splicing events of each locus.
 
  
 
===Module===
 
===Module===

Revision as of 09:27, 25 April 2017

Application Details

  • Description: Bridger is an efficient de novo trascriptome assembler for RNA-Seq data.
  • Version: r2014-12-01, r2014-12-01.1 and r2014-12-01.2
  • Module: bridger/r2014-12-01, bridger/r2014-12-01.1, and bridger/r2014-12-01.2
  • Licence: Github, open source

Usage Examples

Celera Assembler is a de novo whole-genome shotgun (WGS) DNA sequence assembler. It reconstructs long sequences of genomic DNA from fragmentary data produced by whole-genome shotgun sequencing.


Module

[username@login01 ~]$ module add boost/gcc/1.61.0

[username@login01 ~]$ Assemble -h

     ===============================================================================
     Usage: Assemble [--reads/--kmers] <filename>  [opts] 
     ===============================================================================
...

[username@login01 ~]$ Bridger.pl --seqType fq --left reads.left.fq --right reads.right.fq --CPU 6



Further Information

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