Applications/guppy

Application Details

• Description: Local accelerated basecalling for Nanopore data.
• Version: guppy 3.1.5
• Modules: guppy/cpu/3.1.5 (CPU) and guppy/gpu/3.1.5 (GPU)
• Licence: Free, open-source

Usage Examples

Using the GPU as accelerators will make the analysis considerably faster (on large data sets). For small runs the CPU version is recommended and probably faster by removing the GPU overhead.

Batch example

This uses 2 GPUs presently on GPU05 (compute level is high than 6) which is required by guppy's minimum requirement.

#!/bin/bash
#SBATCH -J guppy.job
#SBATCH --exclusive
#SBATCH -o gpu05.%j.out
#SBATCH -e gpu05.%j.err
#SBATCH -p gpu05
#SBATCH --gres=gpu:tesla:2
#SBATCH --exclusive


module purge
module load cuda/10.1.168
module load guppy/gpu/3.1.5


guppy_basecaller --input_path /home/user/fast5 --save_path /home/user/guppy --flowcell FLO-MIN106 --kit SQK-RPB004 --min_qscore 7 --qscore_filtering -x cuda:0 cuda:1

Further Information

• https://community.nanoporetech.com/ (login required)

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