Applications/Rsem

From HPC
Jump to: navigation , search

Application Details

  • Description: RSEM can extract reference transcripts from a genome if you provide it with gene annotations in a GTF/GFF3 file
  • Version:1.3.0
  • Modules: rsem/1.3.0
  • Licence: GNU General Public License v3.

Usage

Prior-enhanced RSEM (pRSEM) uses complementary information (e.g. ChIP-seq data) to allocate RNA-seq multi-mapping fragments. We included pRSEM code in the subfolder pRSEM/ as well as in RSEM’s scripts rsem-prepare-reference and rsem-calculate-expression.

Simulation

RSEM provides users the rsem-simulate-reads program to simulate RNA-Seq data based on parameters learned from real data sets. Run

rsem-simulate-reads

to get usage information or read the following subsections.


rsem-simulate-reads reference_name estimated_model_file estimated_isoform_results theta0 N output_name [-q]


Example

Suppose we want to simulate 50 millon single-end reads with quality scores and use the parameters learned from Example. In addition, we set theta0 as 0.2 and output_name as simulated_reads. The command is:


[username@login]$ module add rsem/1.3.0
[username@login]$ rsem-simulate-reads /ref/mouse_0 mmliver_single_quals.stat/mmliver_single_quals.model mmliver_single_quals.isoforms.results 0.2 50000000 simulated_reads 


Further Information

Icon home.png