Applications/Bowtie2

Application Details

• Description: Bowtie 2 is an ultrafast and memory-efficient tool for aligning sequencing reads to long reference sequences.
• Version: 2.2.9 (compiled with gcc)
• Modules: bowtie2/gcc/2.2.9
• Licence: Open source (Github)

Usage Examples

Bowtie 2 is an ultrafast and memory-efficient tool for aligning sequencing reads to long reference sequences. It is particularly good at aligning reads of about 50 up to 100s or 1,000s of characters, and particularly good at aligning to relatively long (e.g. mammalian) genomes. Bowtie 2 indexes the genome with an FM Index to keep its memory footprint small: for the human genome, its memory footprint is typically around 3.2 GB. Bowtie 2 supports gapped, local, and paired-end alignment modes.

It provides the following tools:

• bowtie2
• bowtie2-align-l
• bowtie2-align-s
• bowtie2-build
• bowtie2-build-l
• bowtie2-build-s
• bowtie2-inspect
• bowtie2-inspect-l
• bowtie2-inspect-s

With linkages to the following modules too:

• Cufflinks
• Samtools
• Tophat
• Samtools
• Bowtie1

[username@login01 ~]$ module load bowtie2/gcc/2.2.9

Further Information

• http://bowtie-bio.sourceforge.net/bowtie2/index.shtml
• http://bowtie-bio.sourceforge.net/bowtie2/manual.shtml

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